Breeding techniques

A mule is half donkey half horse and is sterile like most hybrids.

The closest plant to cannabis is hops. The pollen of the two species is virtually identical, many people have tried to pollinate cannabis with hops pollen and vice-versa.

A few years ago one guy at the lowryder forum showed pics of what he claimed was a lowryder x hops hybrid but it was never verified.

After seeing that I obtained some hops pollen and dusted it all over a cannabis plant, no seeds were produced.

I suppose it would be possible with modern gene splicing techniques to take the gene for THC production from cannabis and insert it into another plant. Maybe the potato, then the term mashed potato would have a whole new meaning! lol
 
A mule is half donkey half horse and is sterile like most hybrids.

The closest plant to cannabis is hops. The pollen of the two species is virtually identical, many people have tried to pollinate cannabis with hops pollen and vice-versa.

A few years ago one guy at the lowryder forum showed pics of what he claimed was a lowryder x hops hybrid but it was never verified.

After seeing that I obtained some hops pollen and dusted it all over a cannabis plant, no seeds were produced.

I suppose it would be possible with modern gene splicing techniques to take the gene for THC production from cannabis and insert it into another plant. Maybe the potato, then the term mashed potato would have a whole new meaning! lol

i thought nettle was cannabis´ closest cousin... anyways, thanks for the reply... your posts are always quite informative

respect
 
"Then they get smashed with metal hot knives"

I suppose it would be possible with modern gene splicing techniques to take the gene for THC production from cannabis and insert it into another plant. Maybe the potato, then the term mashed potato would have a whole new meaning! lol

071129%20advert%20smash.jpg
 
Sounds like someone channelling DJ Short.
N.

Correct Nevil... Thats DJ Shorts hypothisis that I DO NOT agree with. But he sure has alot of folks on the hook about that. There is no proof that this works none..none from DJ or anybody else. Wish he would of never printed that, there are so many folks running around thinking that is the truth..
 
IMO you only need to look as far as the purebreed dogs to see the potential negatives of "bottlenecking of quality" methodology.. just compare the vet bills of a purebred vs a mutt. Why? because when we select heavily for the traits we want inside a small already bottlenecked population linked alleles also come along for the ride many of which are deleterious. I am not sure how much of an issue this is once they are outcrossed again as much of this becomes hidden via heterosis.

I am just really confused why Nevil is apparently against using multiple parents in each generation leading up to the final commercial release. If I grow out Nevil's "typical seedlot" of say 50 AfgTxOrt15 I will very likely find at least three individuals females which have primary traits I am looking for;

-better botrytis resistance (both due to differing structure than regular population and differing outdoor harvest date than rest of population)
-high yielding

All three of these are very similar in these traits but each has a fairly unique effects profile compared to the other.. one similar to sour diesel, one very heavy and hashy and another just different but hard to describe.

I am missing how it is not to a breeders advantage to include all of these individuals in the early stages of an inbreeding program?? As mentioned previously I understand Nevil's reason's behind a commercial release but I believe using multiple parents leading up to the commercial release would be more likely to end in a final 1:1 "bottleneck of quality" than using multiple 1:1 matings in the development phase?? Seems like a win win to me.

Also for the many of us not interested in commercial releases using multiple parents per generation leaves some diversity to hedge against climate/pest issues while at the same time providing some jar diversity in the end product.

If someone can tell me how multi gene traits like flavour, potency and effect are not better carried into the next generation by three parents than one I would like to hear it.

Hi Ortega,

Happy New year.

I am a breeder of dogs and I disagree with you. Unfortunately most breeders of pure breeds don't know what they are doing. But if you have the knowledge and insight to find the best breeders, and you carefully research the breed you are interested in, and you study the pedigrees, with most breeds you will be able to find a breeder(and therefore a dog) that not only will guarantee his pups against genetic defects but will in fact also(more importantly) provide you with a pup that won't have any health problems. I have been breeding my dogs for many years, I have bred many dogs in that time and none of them have had any congenital defects or problems. This is because I researched my pedigrees very carefully, when I started, I only inbred to 2nd or 3rd generation, I made sure that my foundational dogs, were both from champion lines, that had no common ancestors in their pedigree, and I made sure that I established several lines within my kennel starting from the original crosses between foundational Sire and Dam.

I believe the AKC and other kennel clubs around the world are a shining example of why and how "bottlenecking of quality" works. So 90% of the dogs and the kennels they came from are inferior and inbred, but 10% are really good. And the point is: look at the exceptional diversity that can be found in the various breeds of dogs.
Isn't it remarkable? It simply blows my mind. The breeds are simply amazing. Mutts are amazing too, and they are a product of the pure breeds that they stem from.

And that 90% of kennels that are selling shitty purebreeds? Well, if they practiced proper breeding techniques, and they selected their Dams and Sires appropriatly, they would have good dogs as well. But breeding properly, is not simple, it is complicated, and just knowing the info is not enough, you must have good instincts as well, an intuitive knowledge of what constitutes perfect form and character within the given breed you have chosen to work with. Most breeders just don't have either, and as such, probably shouldn't be breeding at all.

What I love about the little information Nevil has disclosed so far in this thread, is that it would allow you to breed weed, as if it were livestock, ie dogs.
And this is truly remarkable, it is like a major piece of the puzzle that I was missing. This concept of progeny testing, 1:1 breeding etc.

You state above:
"I am missing how it is not to a breeders advantage to include all of these individuals in the early stages of an inbreeding program??"

In a 1:1 breeding program you most certainly could include all 3 of those individuals, but you would have to do it in 3 separate breeding cycles, and if you think about it it would be much better to do it that way, even though it would be more difficult and time consuming, because you would know exactly what you ,have and you would have 3 seperate lines.

Now you could start inbreeding those lines separately and independently for several generations, and eventually to prevent negative inbreeding, you could could, after several generations cross those separate lines together, and start fixing traits, and with proper selection, you need never worry about piling up negative alleles. This is what line breeding is all about.

I am seeing now that it is certainly no coincidence that Nevil's strains are the best, the highest quality, and have fewer genetic problems than anything in Holland, or anywhere for that matter.

Keep in mind, negative traits are inherited just like positive traits, and the 1:1 progeny tested pairings that Nevil is enlightening us too, will serve to help us eliminate Negative Traits, just as effectively as it will help us select for Positive Traits.


I for one am on the edge of my seat with anticipation, waiting for the next priceless piece of information that Nevil is going to divulge for our benefit.
 
Can anyone recommenced some good books on plant genetics and breeding? I've read RCC's works. And just picked up a book on Mendel's notebooks.

"Luther Burbank: His Methods and Discoveries and Their Practical Application"

By Luther Burbank.

Essential reading for anyone working with hybrids.
 
In a 1:1 breeding program you most certainly could include all 3 of those individuals, but you would have to do it in 3 separate breeding cycles, and if you think about it it would be much better to do it that way, even though it would be more difficult and time consuming, because you would know exactly what you ,have and you would have 3 seperate lines.

Now you could start inbreeding those lines separately and independently for several generations, and eventually to prevent negative inbreeding, you could could, after several generations cross those separate lines together

The resulting strains are then called Stabilized F1 Hybrids...

Peace.

K.
 
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Can anyone recommenced some good books on plant genetics and breeding? I've read RCC's works. And just picked up a book on Mendel's notebooks.

Selection Methods in Plant Breeding - Izak Bos, Peter Caligari.
 
Here is an old quote that Shanti posted in 2000:

MR NICE BLACK-WIDOW is the F1 of parents you mentioned. However to stabilize the strain it has been back-crossed to both parents as well as three interbreeds. The resulting seed is called BLACK-WIDOW the exact same schedule as white widow.
All the best Shantibaba

This particular quote had been extensively discussed on CW but no one really understood it. People thought that it meant it was an inbred line...
I am sure Hempy and JessE remember.

Peace.

K.
 
People are starting to catch on. Group pollinations are only really practical with highly inbred strains like SK and EP. Many of you will have noticed that most new seed companies start off with a series of SK1 hybrids, basically crossing SK1 to whatever cuttings they can find. It works, but after that they are floundering. After that we enter the realm of...

How to breed with polyhybrids.

Are you paying attention? With polyhybrid matings, it all boils down to the individual. Siblings are not uniform. You see this in the range of types expressed in the females. The males will have a similar range of genotypes. Despite being of mixed blood, individuals still tend to breed true to their own type.

People are polyhybrids. It is quite common for children to look like both parents. Now and again you meet couple who are both singularly unattractive. They produce a ghastly looking baby and ask you, what do you think? I usually say,"It never ceases to amaze me how children capture the essence of both parents in a perfect blend". Nature is like that.

Unless you are doing 1:1 matings with polyhybrids, your results are going to be all over the place. In short, you don't know what you are doing and you'll be unlikely to be able to repeat it unless you keep cuttings of both parents.

I'll get back to this.
N.
 
I've always suspected I was a 'sport' mutant. My dad is 5ft5, black hair, brown eyes, my mum is 5ft, brown hair, brown eyes. I'm blonde, blue eyes and 6ft2, don't look like either parent. However, I am told that I am probably a thrownback to earlier in my dad's genetic line as several of my dad's male forebears were tall and blonde like me. My sister is short and looks like my parents.
 
I've always suspected I was a 'sport' mutant. My dad is 5ft5, black hair, brown eyes, my mum is 5ft, brown hair, brown eyes. I'm blonde, blue eyes and 6ft2, don't look like either parent. However, I am told that I am probably a thrownback to earlier in my dad's genetic line as several of my dad's male forebears were tall and blonde like me. My sister is short and looks like my parents.


lol... what's the mailman look like? heh! I remember coming home from school one day and asking my mom if I was adopted! She said if I was she would have gone looking for my parents years ago. LOL. tough love man I tell ya.

--

Forgive me for interrupting but isn't it possible in controlled conditions to simply introduce a stable male during the flowering stage to pollinate create your lineage? I know you'd have to run the crosses in order to self select your desired traits/phenotypes but either that or painting the females with pollen collected from the males you want to use as sires would make the magical beans right?
 
How do the progeny of poly-hybrid crosses compare with that of true F1 progeny (crossing 2 separate IBLs). I guess the obvious difference is that the true F1s should be more homogeneous but do you get similar advantages with hybrid vigour and good trait expression/combination from the poly combinations or is it a little more hit and miss?

Also, how do F1 hybrids compare - 2 different backcrossed lines combines vs. 2 different IBLs combined?
 
How do the progeny of poly-hybrid crosses compare with that of true F1 progeny (crossing 2 separate IBLs). I guess the obvious difference is that the true F1s should be more homogeneous but do you get similar advantages with hybrid vigour and good trait expression/combination from the poly combinations or is it a little more hit and miss?
Good question, it depends on what you hit and miss. In other words, it depends on the individual genotype of both parents. The pheno of the mother will be known to you, you have picked her as the best. Apart from reversing the males, and this only gives you limited information, you really don't know how they are going to breed. The best practice is to select males that are closest to your female in type, size, branching, leaf structure and smell and resin where possible, and then progeny test each one with your selected female. Tedious I know, but that's the job. If you are trying to stabilise a hybrid, your selections in the early stages with determine the degree of success you have later.
One way of influencing the outcome is by the use of line breeding to certain elements in the pedigree while keeping the rest as an outcross, allowing for some degree of hybrid vigour, but doubling up on key elements.
N.
 
by the use of line breeding to certain elements in the pedigree while keeping the rest as an outcross, allowing for some degree of hybrid vigour, but doubling up on key elements.
N.
I'm not sure I fully understand what you mean here. Are you talking about splitting siblings to inbreed for a set of traits in one line and a separate set of traits in the other line until they are relatively true breeding for those traits then cross them again?
 
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