questions for Nevil

Hi Nevil,
I have a question, I was reading through your posts and I saw a post where you were discussing sensi seeds afghan #1. You had said something about culling everything you had of it. I looked for that thread again and couldn't find it.

The only other Holland strain I have in my seedstock aside from 2 of your original Seedbank strains, is Sensi Seed 94-95 afghani #1.
Not much ever came from that branch for me except a couple of wicked haze hybrids, one of which is about 1/16th afghan #1 and the other is about 1/32 afghani #1.

I have 2 questions

the first is, Why did you cull that seed stock?

the second is, should I be worried about those hybrids I have crossed with 94-95 sensi seed afghan #1, especially the 2 I have just mentioned?
 
Hi pepper,

-regarding nutrients, do you think different nutrient profiles could potentially affect next generations ( seed ) by differing levels of minerals ever? Like feeding a plant to much N over time, next generations will develop a high tolerance to over N???
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This is a really good question! Some time ago I was reading a very interesting paper on 'Transgenerational Epigenetic Effects' that was published recently and found some very interesting info :

"Numerous examples of adaptive maternal effects exist in plants. A range of environmental stimuli acting on the mother, including predation (2), competition (97), soil type (106,107), temperature (68), light (48, 108, 121), and nutrient availability (80, 108, 120, 121), has been found to induce changes to F1 phenotype.

For example, offspring of Polygonum persicaria grown under low light allocate proportionately more resources to shoot growth than those of parents with higher light exposures(121).

Conversely, offspring of limited nutrient plants allocate proportionately more to root growth than genetically similar individuals with nutrient-rich parents (121)."


I would recomend reading the whole paper (or at least the info on plants) as it is really eye opening :
http://www.chd.ucsd.edu/seminar/documents/Youngson.TransgenerEpigenEffects.08.pdf

l33t
 
Thanks for that paper. I would like to get some serious root growth for my outdoor grow. May have to starve my plants to set the seeds up for that. Good find!
 
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the first is, Why did you cull that seed stock?

the second is, should I be worried about those hybrids I have crossed with 94-95 sensi seed afghan #1, especially the 2 I have just mentioned?
Edit/Delete Message
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The Afghani#1 was bred by Sam. When I expressed my disgust at how horrible it had become to Sam, he told me he didn't smoke it. The taste was described by Sam as medicinal. Well it was bad medicine. Let me put it this way. If I hadn't had a smoke for ages, and I found some A#1, I wouldn't smoke it.

Sensi didn't have A#1 for sale in '94. I was making their seeds then. Maybe it was NL1 or Maple leaf x NL1.
N.
 
L33t said:
Hi pepper,

This is a really good question! Some time ago I was reading a very interesting paper on 'Transgenerational Epigenetic Effects' that was published recently and found some very interesting info :

"Numerous examples of adaptive maternal effects exist in plants. A range of environmental stimuli acting on the mother, including predation (2), competition (97), soil type (106,107), temperature (68), light (48, 108, 121), and nutrient availability (80, 108, 120, 121), has been found to induce changes to F1 phenotype.

For example, offspring of Polygonum persicaria grown under low light allocate proportionately more resources to shoot growth than those of parents with higher light exposures(121).

Conversely, offspring of limited nutrient plants allocate proportionately more to root growth than genetically similar individuals with nutrient-rich parents (121)."


I would recomend reading the whole paper (or at least the info on plants) as it is really eye opening :
http://www.chd.ucsd.edu/seminar/documents/Youngson.TransgenerEpigenEffects.08.pdf

l33t
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Very interesting paper, thanks L33t. The arguement of hard genetics versus soft genetics, or Mendelian genetics versus Lamarckism, was thought to be settled over a century ago, it appears that maybe we don't know as much as we thought we did.
While I adhere stringently to principles of Mendelian genetics, as a dog breeder, I have always felt that something more complex is going on. While we have proven beyond a shadow of a doubt, that the Mendelian model is in fact predominantly correct, I do in fact believe that there is also something more subtle going on. Perhaps the theory of epigenetic variation is the answer, or at least a significant step in the right direction. It seems dogs have a plethora of inherited traits, that simply cannot be explained by Mendelian principles of genetics alone. Take retrievers for example, their propensity for retrieving things, ie a stick, is not a learned trait. it is inherited. How does Mendelian genetics or Darwinism explain this?
This is just one small example. In working class dogs there are literally thousands of examples like this, shepherds for example, and Bulldogs. perhaps these traits could have evolved over billions of years, as Darwins theory of evolution through natural selection hypothesizes, but how could these Myriad mutations occur over the space of just a few hundred years?
Artificial selection alone is not enough and by itself is not an explanation in my opinion.
 
Nevil said:
The Afghani#1 was bred by Sam. When I expressed my disgust at how horrible it had become to Sam, he told me he didn't smoke it. The taste was described by Sam as medicinal. Well it was bad medicine. Let me put it this way. If I hadn't had a smoke for ages, and I found some A#1, I wouldn't smoke it.

Sensi didn't have A#1 for sale in '94. I was making their seeds then. Maybe it was NL1 or Maple leaf x NL1.
N.
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I think it was 95. I should have said 94 or 95; maybe even 96. I don't know, a friend of mine brought it back when he went to Amsterdam. He had also brought back some stuff called Hindu Kush and some stuff called Shiva Shanti, but I only got 2 seeds of each and the germination rate was poor, in the end I only wound up with 1 female(afghan #1), and I never saved any males.

I am pretty sure it was Afghani #1, he said that they(Sensi Seeds), had gone to Afghanistan to get it, they brought a bunch of strains back from afghanistan and hybridized them all together to make afghani #1 or something like that. I was not impressed with it at all in terms of smoke(very harsh with a low ceiling), but it had pretty good form and a very short budding cycle (40-45 days) and I used it to shorten the budding cycle of one branch of haze hybrids. It turned out not to be a great cross, but there was one branch of those that I wound up crossing with some of my Malawi Gold Haze hybrids, and they were the only African haze crosses that had a budding cycle under 70 days, or ready by early october in N Cali, which is basically the cut off. The hybrid I am discussing has absolutely no resemblence whatsoever to the initial Afghani #1 in terms of flavor, texture, or effect, so if it is just a matter of taste, I am not worried.

Thanks Nevil, I appreciate the response.
 
old catalogs

hey nevil.. I was lucky here to see the old seed bank catalogs..in mint con I might add.. but that was cool seeing the skunk#1 write up and the g13 was in there.. cool thing to see if you still had them it would be cool to see..
 
Originally Posted by Nevil
SSSc was a competitor. They crossed my stuff with Dutch strains primarily. Their Basic 5 was one of my NL daughters that they grew out from seeds they had sent back from the US or Canada. I can't tell you about their strains, I never paid much attention to what they were doing.
N.




Now dont i feel like an idiot. Sorry Nevil
 
Nevil said:
Sensi didn't have A#1 for sale in '94. I was making their seeds then. Maybe it was NL1 or Maple leaf x NL1.
N.
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When did you stop working for sensi? I ask because about 95 or 96 there early skunk went way down hill... for the longest time we could get 3/4-1 lb per female and after 95 ish it was like the genes were not even the same... never less I stopped buying them around 96...same with the EP@ sensi. Then also in 1996 they ripped me off, I was in Amsterdam buying seeds and went into the store for 300 Early skunks and they had only 2 packs...they said to come back the next day...I did and got something that was not ES...dont know what it was just that it wouldnt finish by the end of sept.. That was the end of me and sensi...forever. The next year I met shanti and got some shark and found a pheno that finished by the end of sept... kept her for 8 years. Untill she was lost in a partner divorce.
 
This may be a question better suited for SB because of your limited involvement in the breeding practices as of late, but do you see any hope for some form of "pure" Northern Lights coming soon?

I'm impressed with the already large variety offered by MNS and plan to start stockpiling genetics soon and I just don't feel that I can trust other banks to stock what they say they do. I'd like very much to purchase some SB & N certified NL genetics.

Do you have any experience with any of the newer stock of NH? I'm curious about how varied the pheno's are of the offspring.
 
Flushing...

Nevil said:
I never flushed. It wasn't practical. With up to 10 rooms, you couldn't run different watering programs. I used to run veg and flower hydro systems, but I couldn't notice any difference if I did veg with flowering formula, so I dropped it.

What you are trying to achieve with flushing is the elimination of plant sugars in the dried product. These affect the taste negatively. If you chop the plant at the base, and slowly dry the whole plant, it will take at least a couple of days to fully die. In this period the plant uses the remaining sugars.

If conditions were humid, I'd remove the fan leaves, but I preferred not to. If the plant is hung upside down, the leaves dry around the bud offering natural protection. When you smoke it, it's still in pristine condition. I just hate it when people manicure wet and smear the resin around and then force dry it. That's just disrespecting the plant. I like to manicure as I'm preparing my joint. It's the natural way.
N.
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N to the S,

These are gems man... little pieces of gold flying all around this place...

It seems you are not the only one that believes this theory...

I was doing some diligence with regard to having analysis done on my cultivars and came across an article in Cannabis Culture dated October 2005 with Dr. Paul Hornby. Here is the link if anyone is interested: http://www.cannabisculture.com/v2/articles/4552.html but I was hoping to just reference the paragraph where he explains why he subscribes to your though on flushing and your words hit a note:

"Well that?s because you?re flushing. And when you flush, you stop your nutrient flow into your plant; it quits producing resinous cannabinoids, your leaf weight increases into the last week, but you don?t see the cannabinoids increase. You?re diluting your cannabinoids in the leaf weight. But if you cut it at week seven, you?re going to get the nutrients in your bud. There?s a catch-22 there. We?re trying to sort out which nutrients are required to keep the THC coming while the flush is continuing, if that?s possible. So, this is news for all growers. We discovered this phenomenon about a month ago. We?ve seen it before ? it just hadn?t really come clear in our minds."

Nev care to share some more insight into it. I have always been to scared not to... I always believed I would ruin my crop not flushing...

Cheers,

CM
 
Use molasses since its a chelating agent the plant will use up all nutrients available and its high in potash which will make them calyxes swell and finish a little bit faster. I like to catch my pk spike near the end of flower thats why i use molasses in the last 2 weeks of flower i find it a saviour!:D
 
GUANOIZDASHIZNIT said:
Use molasses since its a chelating agent the plant will use up all nutrients available and its high in potash which will make them calyxes swell and finish a little bit faster. I like to catch my pk spike near the end of flower thats why i use molasses in the last 2 weeks of flower i find it a saviour!:D
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Good day Guanoizdashiznit, thanks for the suggestion. Any word on the ec (ppm) for the last two weeks with regard to using the molasses?

Cheers,

CM
 
Great post Chana. Dr. Hornby makes a good point as to why cannabinoid content should be analyzed using high pressure liquid chromatography as opposed to gas chromatography when he states that the high temperatures used in GC will convert one cannabinoid to another (ex: He mentions THCA -> Delta-9-THC).

I would like to see more research into terpenoid content and how they affect the effects of particular cannabinoid profiles when consumed. I don't know how relevant this is to medicinal cannabis research but it certainly seems relevant to recreational usage! I often think of the combination of light crystals and their multitude of effects in the "Land of the Lost" when I think of the influence of terpenoids/cannabinoids on the high.

Good read.
 
Nevil said:
The Afghani#1 was bred by Sam. When I expressed my disgust at how horrible it had become to Sam, he told me he didn't smoke it. The taste was described by Sam as medicinal. Well it was bad medicine. Let me put it this way. If I hadn't had a smoke for ages, and I found some A#1, I wouldn't smoke it.

Sensi didn't have A#1 for sale in '94. I was making their seeds then. Maybe it was NL1 or Maple leaf x NL1.
N.
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couldnt agree more sensis A1 was truly crap..one decent pheno,harsh-yup.low ceiling/legs -yup..

got their juicy fruit around the same time (late 90s) that was a dud too.

I have tried a good portion of most A-dam genetics and the only ones I can say sucked outright where those. .

I had some nice maple leaf in 94 along with early and silver pearl and durban..all where on the money.

late 90s went to shit for them IMO and the customer service ,in the shop,sucked ass.Total bullshit.
 
In regards to flushing and cutting. Nev stated that he cuts the plant at the base and drys the whole plant. I have heard of grower up rooting the plant instead of cutting it before drying is there an advantage to this technique? Or is this more of an old wives tail?
 
drying

I belive this is an old wifes tail back in the old days I remember growers up rooting thier plants and boiling the roots to make the thc from inside the plant to force it to the outside but this is a myth because there is noTHC on the inside . I also cut at the base and dry I dont think there is any advantage to up rooting you plant
 
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Nevil said:
I'd like to answer all of your questions, but I am a reluctant one finger typist. If we were sitting around having a smoke you could ask me anything you want. I will limit myself to what I think is important. I'll finish the haze story yet because I can imagine that You think it's important. I would if I were you too.

What I think is important, is that we have some very old seeds, with some rather exclusive genetics and we have to save what we can. The most promising lead that I could come up with is this:- http://blip.tv/file/3881270/
I'd like you to look at this and tell me what you think. If you have other idea's how to pump some life into old seeds, I'm willing to entertain them. Maybe we need to sacrifice some virgins or something. (just joking)
N.
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I think Tissue Culture is the answer your looking for.
 
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