Freezing Seeds

[lozac123]

You all might find this interesting:

A Belated Green Revolution for Cannabis: Virtual Genetic Resources to Fast-Track Cultivar Development - PMC

Cannabis is a predominantly diecious phenotypically diverse domesticated genus with few if any extant natural populations. International narcotics conventions and associated legislation have constrained the establishment, characterization, and use ...

pmc.ncbi.nlm.nih.gov

"Common practice for short-term seed storage is 4°C with a moisture content of 6% (De Meijer and van Soest, 1992; Small and Brookes, 2012; Mankowska and Silska, 2015), while for longer periods of storage >3 years, seeds are held at −20°C with ~4% moisture content (De Meijer and van Soest, 1992; Mankowska and Silska, 2015). Cannabis seed appears to have orthodox storage behavior, and the ability to withstand periods of up to 66 months after desiccation with minimal effects on seed viability (Small and Brookes, 2012; Parihar et al., 2014). Nevertheless, systematic evidence for the long-term viability of Cannabis seed is lacking."

 

[longball]

I remember reading this 10 years ago when it came out. All information is 10-40 years old and has been mentioned in earlier posts.

"while for longer periods of storage >3 years, seeds are held at −20°C with ~4% moisture content (De Meijer and van Soest, 1992; Mankowska and Silska, 2015). Cannabis seed appears to have orthodox storage behavior, and the ability to withstand periods of up to 66 months after desiccation with minimal effects on seed viability"

This says the same thing Svalbard says/does - dry your seeds before freezing(desiccation), this is a few hundred dollars(USD). CyroGram may store your seeds $500USD for 5 years(renewable, with a price rise).

For the rest of us, storing your seeds, airtight, at 0F/-18C, will work for 10-20 years. No desiccant needed. You may use a desiccant at this temperature but your seeds won't stay viable as long.

Also, no, my freezer does not ice up. In this case you may need desiccants but that's just a band-aid. You don't have desiccant problem , you have a freezer problem.

Seeds stored in containers on metal shelves in Svalbard.



Aluminum foil packages of seeds stored in container at Svalbard.

 

[PennyP]

Greetingz and Blessing to all of ya,

It seems ro be a great discussion how to do the right way....i Just want to expand the Live-time of the Seeds I bought from various Seedbanks over the Last month and Years ...so i think there were at a right Level, when i bought them.... Might be different from the Seeds i produced myself....

After all i read hear i will make an experience for my self and Store them in my freezer . Put the Seeds in the little Container with some Cotton. The Container will be airtight.....then Put them in a ziplocker an the zipback in a Tupperware Container with some Rice corn in there ....and that Box in the freezer at -18°c.

If there will hold vigor for the next 5 to 10 Years....i am Happy and thank Jah....If Not...
I get me some new from Shanti or other cool Breeder...

Seems to me better, than to Store em in der fridge in the next Years....

One Love and thx for all the Input

Penny

 

[Ken Abbis]

The extremely short reason of using desiccants is it is like using a condom when the girl you're with is already on the pill. I really don't want an unplanned kid, id rather the double protection.

If the girl your with is on the pill, she doesn't really like you lol
If you're wrapping your purple headed custard shooter with a condom, you don't really like the girl your with.
So what's the fucking point of that?
You end up getting drunk, horny AF, she forgot to take her pill, you can't find a rubber and here comes along joe unplanned bastard ffs.
You end up separating because you never really liked, or loved, each other.
Poor kid.
Ken Abbis Top Tip of the day: Never fuck anyone you wouldn't have children with.

 

[Wobby]

Ken Abbis Pro Tip of the day: Never fuck anyone you wouldn't have children with.

Make a good signature

 

[longball]

After all i read hear i will make an experience for my self and Store them in my freezer . Put the Seeds in the little Container with some Cotton. The Container will be airtight.....then Put them in a ziplocker an the zipback in a Tupperware Container with some Rice corn in there ....and that Box in the freezer at -18°c.

If there will hold vigor for the next 5 to 10 Years....i am Happy and thank Jah....If Not...

Been doing something like that for 7 years now. 100% germination rate.

Over the centuries, the same 3 things destroy plants/meats/seeds that are being used for long term storage - oxygen, heat, light(esp. UV)
This is all covered under Issac Newton's Second Law of Thermodynamics. Airtight packages, protected from light, and stored at -18C takes care of this.

Before freezers, people stored their perishables in a hole dug under their cellar called a 'root cellar' - it was dark, it was cooler, and there is less oxygen there. Ta Da!
The small amount of oxygen there was fine for the exchange of gasses in ripening vegetables/fruit.

Instead of ziploc bags which let a good amount oxygen back in very quickly - consider vacuumed sealed mason jars or vacuumed sealed bags? If not possible than a ziploc is better than nothing.

 

[Ken Abbis]

Make a good signature

If the local courthouse tries to make me sign anything in the future I will sign the documents with it.

.....This is all covered under Issac Newton's Second Law of Thermodynamics. Airtight packages, protected from light, and stored at -18C takes care of this.

Isaac Newton thought he was God. Maybe he was/is?

The First Law of Thermodynamics
Energy cannot be created or destroyed but is just transformed from one form into another.

But Genesis says God created everything.... or did God (Isaac) just transfer us here from another Matrix?

I bought small foil seed packs, like what commercial seeds come in. can get around 2gram of seed in each. Squeeze the air out and seal with vacuum sealer..

leave your seeds at room temp for about 6 months before you bag em up.

Before freezers, people stored their perishables in a hole dug under their cellar called a 'root cellar' - it was dark, it was cooler, and there is less oxygen there. Ta Da!

think i seen that done in Himalaya they were stashing butter underground

 

[Monkey.D.Luffy]

Hi have you ever heard about diamond painting ? Crystal Art Storage Cases are Ideal for seed storage.

 

[musashi]

While the above is more appropriate for bead storage, I don't think it is suitable for long term fridge or freezer. Mason jars with rubber seals are more up to task and vacuum sealing them ensures seeds are stored at there predetermind moisture content. I use small labeled amber vials that will accommodate the individual seed straws the seeds come in from auction. I do put several grains of rice within the bottles. I then put these vials into a mason jar that I vacuum seal with my Vacuum Seal 2840 or I use a Pump-n-Seal. I've pulled seeds from 2014 that have a 95% germination rate.


mu

 

[musashi]

Svalbard is a facility that stores for end of the world scenarios, I'm not sure we need to go that far. Though I do wish I would have stored my Skunk seeds from the 80's in a better fashion

Hi All

I prefer to keep seed in the fridge at between 3-5C, if you keep them in that situation you will be able to keep them for some years...

If you freeze seed you will not be able to refreeze it so be prepared to use it after this one time storage method. Breaking larger batches up into smaller ones is a good option for this method also.

Heat and direct sunlight are just two methods that reduce the germination levels by basically cooking the embryo inside the seed capsule. You can tell nothing from the seed as it looks fine but inside has been killed. Irradiation is just one way the food industry treat things to preserve them longer...it is for me a very bad option but it does give shelf life a little longer. The agricultural industry has used it in Holland to erradicate exporting bugs and eggs via flower/plant export...in a limited way. If this irradiation occurs to seed it is killed, again without visible signs. The use of Kirilian photography is something we at MNS have dabbled with a little.It seems to be one of the safest ways to tell if the embryo inside the seed case is alive or dead. So maybe in the future we will impliment this to scan the seeds for viability.Till then you will need to use the fridge or freezer....all the best Sb

Hi All

well since I just sent some seed recently to a chap from 2002 stock that had been kept in a fridge all its life...and had very good germination levels I believe the methods of long term storage are as follows....

1. We keep 2 stocks of seeds, i for the everyday use and 1 for long term use.
2. Long term use means vacuum sealing the seed in small quantities in an air tight , moisture resistant plastic bag and kept in the freezer.
3. Short term use is keeping seeds dry in air tight containers, always put back in fridge when not in use.
4. Point about long term stock you cannot defreeze seed only one time and then keep it is the fridge stock, till it is used.

We have seeds from 30 or 40 years ago still in fridges and even if germination is lower it still pops up a few ...so it all works for us.

Be well all the best sb

mu

 

[charlesufarley]

While the above is more appropriate for bead storage, I don't think it is suitable for long term fridge or freezer. Mason jars with rubber seals are more up to task and vacuum sealing them ensures seeds are stored at there predetermind moisture content. I use small labeled amber vials that will accommodate the individual seed straws the seeds come in from auction. I do put several grains of rice within the bottles. I then put these vials into a mason jar that I vacuum seal with my Vacuum Seal 2840 or I use a Pump-n-Seal. I've pulled seeds from 2014 that have a 95% germination rate.
View attachment 108536

mu

Seeing that immediately reminded me of this :

Here's what I use to get a good vacuum with canning jars:

 

[Phineas Phreak]

I keep my seeds in the freezer inside 5ml "cryo tubes" (just a fancy name for what is basically an air tight plastic tube) with silica
gel beads inside.
I put the seeds at the bottom, then a cotton filter on top and then silica gel beads up to the brim and leave them at room temperature.
The beads will change color after a couple days due to having absorbed some of the moisture inside the tube plus what is the cotton filter. Then I open the tube, dump the humid beads out (seeds remain at the bottom of the tube protected by the cigarette cotton filter) and replenish with more fresh silica gel beads, screw the cap again and leave for a few more days at room temp, and they never change color, indicating the moisture inside is now low. Then I put them in the freezer, where they can stay in this condition for many many years, even decades, although I have only tested 5 year old seeds and got 90+% germination rate.

 

[easty]

If the girl your with is on the pill, she doesn't really like you lol
If you're wrapping your purple headed custard shooter with a condom, you don't really like the girl your with.
So what's the fucking point of that?
You end up getting drunk, horny AF, she forgot to take her pill, you can't find a rubber and here comes along joe unplanned bastard ffs.
You end up separating because you never really liked, or loved, each other.
Poor kid.
Ken Abbis Top Tip of the day: Never fuck anyone you wouldn't have children with.

Ha ha ha this advice should be given to kids in highschool

 

[Ken Abbis]

Ha ha ha this advice should be given to kids in highschool

School would be so much easier for everyone if it was High AF School.

then there's this guy....
Not your great grandfather @Lu-Khan ?









these are the seed foil bags I use... 5x7cm... use a small funnel to get the seeds in the bag. they roll up well and push out excess air... seal with heat sealer on vacuum sealer, no vacuum required. Seen lot of commercial seeds in these usually dated to sow within 3 years of packaging and they all at room temp. All mine went in freezer after 6 month room temp air dry. Expect them to be viable in 30 years but will test viability over the years and report back.

100pcs Silver Dumb Aluminum Foil Bags Heat Seal - Top Open Mylar Foil Bag Matte Surface Plastic Food Package Powder/Sauce Pouch
https://www.aliexpress.com/item/32664684853.html

 

[easty]

School would be so much easier for everyone if it was High AF School.

then there's this guy....
Not your great grandfather @Lu-Khan ?


View attachment 108546

View attachment 108547

View attachment 108548

View attachment 108549


these are the seed foil bags I use... 5x7cm... use a small funnel to get the seeds in the bag. they roll up well and push out excess air... seal with heat sealer on vacuum sealer, no vacuum required. Seen lot of commercial seeds in these usually dated to sow within 3 years of packaging and they all at room temp. All mine went in freezer after 6 month room temp air dry. Expect them to be viable in 30 years but will test viability over the years and report back.

100pcs Silver Dumb Aluminum Foil Bags Heat Seal - Top Open Mylar Foil Bag Matte Surface Plastic Food Package Powder/Sauce Pouch
https://www.aliexpress.com/item/32664684853.html
View attachment 108550

I know for a fact I went to High AF school, I barely remember my last 2 years

 

[Lu-Khan]

@Ken Abbis
I completely agree with all of his tips, so there's definitely a connection between us

 

[Monkey.D.Luffy]

Diamond painting cases can also function as a primary preservation layer reasonably well, especially when combined with simple moisture control (rice and cotton, as already suggested).
The main idea isn’t to replace proven longterm methods, but to highlight how useful these cases are for organisation and seed handling. Smaller sealed vials already, mentioned, can easily be stored inside them, so both approaches can work together rather than being mutually exclusive.
In the end it comes down to use case: maximum long-term preservation vs. practical organisation.
A layered approach covers both, and the tools included in these sets are genuinely useful.
Useful items included in this diamond painting kits:

• Small labeled plastic containers-individual separation and easy identification
• Mini funnels-clean transfer of small seeds without spills
• Tweezer-useful for removing debris and handling
• Sorting trays-temporary holding, counting, and cleaning
• Storage case with compartments-keeps everything organised (including pockets for tools and new seed packages)
• Label stickers / number tags-clear cataloguing and tracking
• Small scoops or pens-controlled movement of tiny items
• Seal caps or screw lids-basic dust and moisture protection (short-term or inner storage)

 

[musashi]

I thought you made your suggestion earlier? You are now sounding like an advertisement. This post is about freezing and storing the frozen seeds preferrably in air tight containers.

 

[Monkey.D.Luffy]

I'm sorry If my post made you feel that way.
No It wasn’t an advertisement, just a direct response to your concern about long term fridge or freezer storage. I described more in detail for ones that might find it helpful and ones might didn't understand.
Nice to meet you.

 

[PennyP]

Greetingz and Merry Christmas to all of you in the Community,

It might be a little bit of topic but the question goes in the Same direction.

What is the best way to Store Male Pollem for further Projects?

We have a small pollintation going on, and the very potent Male is still producing a huge amount of Pollem.

What can I do to keep the pollem usable for a Period of lets say the next three months to selectivly Put some of his Powder to a small amount of female Flowers later on?

The Male in the Back is an Bubble Gum Bx2 from Fleur de Mal Seeds....very Early and is already bringing Male Flowers under 20h of Light, thats the reason we chosed em for the Project (the smell of the stem rub is amazing too)....to make the Bubble Gum F1 and the other Bubble Gum x Bubble Gum BX3 to flowering earlier Outdoor.