For any history buffs who might be reading this, and hopefully your voracious readers as well because there's a _lot_ of info on this page, you'll see how Mr Soul learned to develop C99. I was a fairly active participant in this discussion, just not as Charles U. Farley.
Hey charles, thanks very much for sharing that very helpful link. Wow, you weren't kidding about the wealth of information on that page. BOY, it's some solid gold. I'll download and save it, as I'd hate for that site to go down, and that info to be lost.
I don't know much about Vic High but I have to wonder if he had a degree in botany & was a professional horticulturist / plant breeder, by training; not just a cannabis breeder. It will take me some time, as an amateur, to really penetrate the true depth of what Vic shares there.
One distinct impression it left on me is that there doesn't seem to be a lot of cannabis "breeders" really doing it right; due to the amount of work it actually involves. If they followed established breeding principles, (as Vic implies,) there wouldn't be nearly as many different strains on the market today, as there currently is. He states the following, for example:
"Most serious breeders learn to take small methodical steps and work on one or two traits at a time. Especially with inbreeding projects such as selfing and backcrossing."
So, if a breeder is working on just a single trait at a time, cubing (or quadrupling) the backcrosses, for that trait, it should take a very long time to complete the project; possibly a decade, or two. It's not hard to see how a breeder could easily dedicate 20 years to perfecting a single strain, using the old tried & true method(s). However, with the lab work available today, to breeders, (as Jodrey mentions in the video below,) the process can be considerably expedited.
Lady J comment on Nevil's breeding practice, states the following:
"Nevil backrossed six times to the earliest son before he called a strain stable. That's what he did to create nl#5 which was only a clone he got from the U.S. But more importantly, this was just the starting point. Once the sixth backcross is complete, the best indivuals both male and female were selected to start at least two separate lines, neither of which was the original plant. This was the p1 generation."
Not sure how accurate Lady J's comment is, but maybe you or Musashi could verify its accueacy for us. I found it to be very interesting, though I'm not quite sure how to understand her last sentence. Sounds like she says that 4 parents selected from the BX6, to start 2 parallel IBL lines from, are designated as the P1 generation.
She says Nevil didn't back-cross to the elite clone mom, but only to her son(s); which is a mystery to me. Is greater stability (& true breeding) achieved by not backcrossing to the elite clone mother?
According to Vic, though, "if the backcrossing continued indefinately with random selection of males and with large enough of a population size, the frequency of the P gene would max out at 50%. This means that the best that can be expected from cubing is 25% true breeding for," a single trait.
So, that raises the question about the possibility of increasing that true breeding "percentage" by further, more advanced methods of inbreeding? Lots of questions and things to discuss, on that golden page, but this isn't the appropriate thread for that exchange
Even after reading that page twice, I'm sure I'm barely scratching its surface.
Cheers! - FF
Kevin Jodrey: How I hunt breeder males.