The Resuscitation, Revival and Resurrection of Old Heirloom Seeds

I lost the best strain I ever saw to the feds, in 1997. I grew it for almost 7 years, and a partner ratted me out. It was so strong, one girl is still mad at me, because she swears I put PCP in the joint.

I had given some seeds to a buddy, in 1996, and he grew for 4 years, and stopped, in 2000. He just informed me, he has about 1000, or more of the seeds. They have been kept in a garage, that doesnt get above 68f, or below 60f. They have been in a film cannister. He lives 100 miles away, but will soon come into town in a couple months, and give them to me.

Ive been trying to find someone that is an expert in Embryonic Tissue Culture Rescue. They rescue the seeds. Make seeds, and give me, and my buddy some seeds, in return.

Ive spoken to Katsu about it, as he is starting to get into tissue culture, but told me to wait, as he needs more experience, and hasnt done Embryonic Rescue yet. Just normal tissue culture.

Ive also spoken to a guy named Marcus Aureolus. On Instagram. He is in a partnership with people that do this, but says its not his call.

The strain is/Hybrid.

NL5 x Haze x Nevils Hashplant x Skunk #1. Truly the best stuff Ive seen for indoors, and top 3 of all time. Not saying Ive seen everything, but Ive been smoking, since 1967, and have seen some of the best of the best of the old landraces. And this stuff, compares to any of them. Is done in 7 weeks inside, but 1st week of November, outside. Its Narrow Leaf. REEKS of flowers, skunk, and hash. And not only was the buzz A#1, the taste, wawas to die for. Tasted like the very best floral hash. And was total lung buster. You knew when you smoked it, you were going to cough. Itwas also unsafe to try and drive, and smoke it. It could make you lose your vision, for several seconds, and get the headrush from hell. Dizzy. Every hit. No Ceiling. Could be a very anxiety, and paranoia inducing strain. And a very heavy physical high. I lost customers, because they said it made them paranoid, and they wanted to hide under the bed.
 
59lespaul said:
NL5 x Haze x Nevils Hashplant x Skunk #1.
Click to expand...

Very interesting! Would love to smoke that. Perhaps crossing the MNS NL5×Haze with G13×Skunk#1 will reproduce something very similar.

Here's a guy on YouTube that teaches tissue culture courses online. I've been thinking about joining his program:

Keeping all my seed sterile and efficient || Meri Genetics

Meri🌱Genetics Tissue Culture Talk

Cheers! - FF
 
59lespaul said:
I lost the best strain I ever saw to the feds, in 1997. I grew it for almost 7 years, and a partner ratted me out. It was so strong, one girl is still mad at me, because she swears I put PCP in the joint.

I had given some seeds to a buddy, in 1996, and he grew for 4 years, and stopped, in 2000. He just informed me, he has about 1000, or more of the seeds. They have been kept in a garage, that doesnt get above 68f, or below 60f. They have been in a film cannister. He lives 100 miles away, but will soon come into town in a couple months, and give them to me.

Ive been trying to find someone that is an expert in Embryonic Tissue Culture Rescue. They rescue the seeds. Make seeds, and give me, and my buddy some seeds, in return.

Ive spoken to Katsu about it, as he is starting to get into tissue culture, but told me to wait, as he needs more experience, and hasnt done Embryonic Rescue yet. Just normal tissue culture.

Ive also spoken to a guy named Marcus Aureolus. On Instagram. He is in a partnership with people that do this, but says its not his call.

The strain is/Hybrid.

NL5 x Haze x Nevils Hashplant x Skunk #1. Truly the best stuff Ive seen for indoors, and top 3 of all time. Not saying Ive seen everything, but Ive been smoking, since 1967, and have seen some of the best of the best of the old landraces. And this stuff, compares to any of them. Is done in 7 weeks inside, but 1st week of November, outside. Its Narrow Leaf. REEKS of flowers, skunk, and hash. And not only was the buzz A#1, the taste, wawas to die for. Tasted like the very best floral hash. And was total lung buster. You knew when you smoked it, you were going to cough. Itwas also unsafe to try and drive, and smoke it. It could make you lose your vision, for several seconds, and get the headrush from hell. Dizzy. Every hit. No Ceiling. Could be a very anxiety, and paranoia inducing strain. And a very heavy physical high. I lost customers, because they said it made them paranoid, and they wanted to hide under the bed.
Click to expand...

If he got 1000 or more seeds andbthey have been stored the way youbsaid you'll probably have enough popping for a preservation without any TC involved.
 
Here is a step-by-step explanation of the Embryonic Tissue Culture Rescue process:

  1. Seed Collection:
    • Collect seeds from the desired plant species.
  2. Surface Sterilization:
    • To eliminate any external contaminants, the seeds are often surface-sterilized using disinfectants such as ethanol or sodium hypochlorite.
  3. Dissection of Seeds:
    • Open the seeds carefully to expose the embryos. This step is crucial when dealing with seeds that have hard seed coats or when embryos are not easily accessible.
  4. Isolation of Embryos:
    • Isolate the embryos from the rest of the seed components. This process may involve dissection under a microscope to carefully extract the embryos without damaging them.
  5. Culture Medium Preparation:
    • Prepare a suitable culture medium that contains a balanced mix of nutrients, vitamins, and growth regulators necessary for embryo development. The medium is usually agar-based and may include plant hormones such as auxins and cytokinins.
  6. Placement of Embryos on Culture Medium:
    • Gently place the isolated embryos onto the prepared culture medium. The embryos should be positioned in a way that allows for their growth and development.
  7. Incubation:
    • The culture dishes or containers with the embryos are then placed in a controlled environment, typically in a growth chamber or incubator. The temperature, light conditions, and other environmental factors are optimized for the specific plant species.
  8. Observation and Subculturing:
    • Regularly monitor the cultures for signs of growth and development. Subculture the embryos onto fresh medium as needed to provide them with the necessary nutrients and space for continued growth.
  9. Rooting and Acclimatization:
    • Once the embryos develop into seedlings, they may be induced to produce roots on the culture medium. After rooting, the plantlets are carefully acclimatized to normal growing conditions by gradually exposing them to external environmental conditions.
  10. Transplantation:
    • Finally, the successfully rescued and acclimatized plantlets are ready for transplantation into soil or a suitable growing medium for further development into mature plants.
 
Yeah, this thread is about techniques to revive old seeds. Any input on OP's topic is welcome and appreciated. It is primarily my thread about reviving my '80 skunk seeds. Posts about inventory/seed availabilty or other off topic posts are not appropriate here. I am happy to relocate your post to someplace more suitable.

mu
 
It has been 1 week since the first batch of seeds were put to soil. I'm sorry to say, nothing has emerged in 24/0 light, 78 F. As I said, it is a journey of miracles. 50 more seeds cracked this morning to be soaked in my regular hatching juice of kelp and fulvic acid to 6.0 pH, then submerge 24-72 hrs. Replant in existing pots and start the count again. If I get no results then I will table this project for now and resume the regular growing of MNS gear. I have been smelling Master Kush lately though I thought I saw some EQ x AfghanHaze the other day ;) Glad to have lived in the Emerald Triangle when this strain was the "thing" back then. Having been in the thick of it, I am grateful for the memories but I do not live in the past. There are so many options today that are much better IMO. Onward!

50moresk.jpg50seedsoak.jpg

mu
 
musashi said:
It has been 1 week since the first batch of seeds were put to soil. I'm sorry to say, nothing has emerged in 24/0 light, 78 F. As I said, it is a journey of miracles. 50 more seeds cracked this morning to be soaked in my regular hatching juice of kelp and fulvic acid to 6.0 pH, then submerge 24-72 hrs. Replant in existing pots and start the count again. If I get no results then I will table this project for now and resume the regular growing of MNS gear. I have been smelling Master Kush lately though I thought I saw some EQ x AfghanHaze the other day ;) Glad to have lived in the Emerald Triangle when this strain was the "thing" back then. Having been in the thick of it, I am grateful for the memories but I do not live in the past. There are so many options today that are much better IMO. Onward!

View attachment 82702

mu
Click to expand...
Interesting, I’ll have to look up fulvic acid.
Hope it works out for you
/steve
 
Hi Mu,
Ive had some good success doing what you are doing with stratification and the kelp + fulvics soak.

My only suggestion could be to add an extra step between the kelp soak and putting them in soil.
I feel like you can easier mitigate potential dry back within the soil, and other inconsistencies of soil from this point by instead heading to "the paper towel method":

1. Some people use paper towels.. a better option is those thin chux surface sponges. Cut two squares large enough to hold your seeds spread out ideally not touching each other.
Soak in fresh (pH'd) warm, not hot water only (u can use a light addition of h202 in your soak too)

2. Use tweezers (avoid fingers, or at least get some nitrile gloves on) to transfer your seeds from the soak solution to your soaked sponges, lightly sandwich the seeds between the two sponges.

3. Put sponges into a ziplock bag and seal, store this in a cupboard (hotwater cupboard over winter) - place a plate or bowl over top to keep potential rodents away.

4. Check seeds every day, and keep them within the sponges untill tails start showing.

Once tails show - then, using tweezers, carefully plant into seedraising mixes.

After 7 days if tails are not showing - refresh the sponges in a new soak, and persevere another 7 days before considering discard.
It can be surprising but some old beans just take a little longer than normal to pop.
 
Well braddahs, sorry to say my second attempt using a different germination method ended in failure as well. 100 seeds- no más. I am a little disheartened but I do have more seed and will try the Coots method and then a GA method. If those don’t work then kick the dog and spit on floor, I’ll head for the freeze to choose from many other varieties. Abundance and choice, it sure wasn’t like that back in the 80’s.

mu
 
musashi said:
Well braddahs, sorry to say my second attempt using a different germination method ended in failure as well. 100 seeds- no más. I am a little disheartened but I do have more seed and will try the Coots method and then a GA method. If those don’t work then kick the dog and spit on floor, I’ll head for the freeze to choose from many other varieties. Abundance and choice, it sure wasn’t like that back in the 80’s.

mu
Click to expand...
Bugger. Try some water from sprouted barley as a last resort? Lots of good enzymatic activity and carbohydrates, in it, ya never know.
 
musashi said:
Well braddahs, sorry to say my second attempt using a different germination method ended in failure as well. 100 seeds- no más. I am a little disheartened but I do have more seed and will try the Coots method and then a GA method. If those don’t work then kick the dog and spit on floor, I’ll head for the freeze to choose from many other varieties. Abundance and choice, it sure wasn’t like that back in the 80’s.
Click to expand...
Sorry to hear that none of them popped. Like you said, at least you have a plethora of amazing choices.
 
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