Fem seeds and there impact on the future of the species known as cannabis.

[kylebroflovski]

Prop 19/Monsanto Thread
http://www.mrnice.nl/forum/showthread.php?t=4574

See if there is anything in this thread that would raise any red flags. I see a couple of causes for alarm. Later brother.

KB _~

 

[GreenintheThumb]

GitT- "I thought this thread was about gynoecious cannabis?"

NO. Gynoecious cannabis (all female flowers) is not the ONLY topic being discussed. This is a very long thread, try starting from the first page. We are also discussing POTENTIAL genetic problems which could arise from the feminized seed club, such as the rise of intersexed populations. We also touched on the fact that certain survival genes might be found only on the Y chromosome. We are covering MANY of the points about the threat of feminization.

Okay, the rise of intersexed populations will only occur if you're making bad selections. Hormone treatments are NOT making selections towards intersexuality unless you don't stress test you parents. Something everyone should be doing anyway.

On the issue of certain survival genes being ONLY on the Y. Well this is pretty much a joke. Studies show there's a PAR region on cannabis's Y. So we know at least parts of it recombine normally. And the part that is truely male, well if that had "survival genes" I guess the species would have died out a long time ago.

GitT - "I'm not sure how it applies to twins. But for the record I've seen monozygotic twins in cannabis populations. And I only WISH I've ran Sam's numbers"

The point you conveniently be seeming to miss is the coorelation between haploids and twins. Sam S is doing haploid experiments, you guys at icMAG are having lots of twins. See the connection? FYI i have observed an increase in these phenomena over the last decade (but NOT in MrNice seeds) I'm sure it's just a coincidence. 8/

I'm also sure it's a coincidence. Ya know, cuz it's happened forever and in all sorts of different species. I guess I'll tell my buddy who's a twin his parents must have been messing with Sam's evil haploid experiments. Surely there were no twins on this planet before evil Sam started tinkering with the frankenweed.

 

[Nevil]

Greeninthethumb, Sam, who ever the fuck you are, what are you doing here. The thread is full of failure. Are you here to insult the "uneducated idiots" or do you seek approval from those you label as ignorant? You come here with an elitist attitude, a bag full of fancy terms singing praises of the biggest traitor in cannabis history. You're not dependent on closet amateurs for your germplasm and you're excited by what huge cooperations are up to. There's no big corperations here. Why don't you go a impress Monsanto with your brilliance.
Wether you are Sam or not, your mindset is exactly the same. I remember the first conversation I had with Sam... Double factor, you mean homozygous. Pompous prick.

Do you think giving a fancy term to a commonly understood concept makes you a better breeder? Don't kid yourself. Even the big corporations like Monsanto can't equal the results of selective breeding. Like you, they're a fraud. You can't turn your back on you're own kind and then expect their admiration. Piss off!
N.

 

[hempy]

I do care what the people who visit this thread think about gynoecious releases. Their closets might not make it into my personal collection but they end up in the pool at large and its health is of utmost concern to me.

Your kiding right fem seeds being pumped out for 6 years now from people that are fucken hacks that people have grown to show hermis and males dont bother you or worrie you a little od if your honestly about the species called cannabis.

Most wont give out there fem proces in short on how they make there fem seeds GA has been used and i bet still used by most and research has found it lowed THC in the plant what type of improvement would you call that.

They use aspren / gibberellic acid / and other chemicals yet they have no idear on how it affects cannabis but all they care about is makeing seed that is the jest of it.

Lets get down to the facts here these people cant breed they dont have the plants that made these lines so they can not reproduce them so they get hold of f1 seeds from shanti and a few others that in fact offer reg seeds and select a good fem and make seeds for market thats it in short.

They are to lazy to grow out seed select a fem they like then select out males to x to her to then test and see which of the males will combine the best to then go on with in short there not wonting to do it as it was ment to be done and how it has been done for 1000s of years they wont the easy lazzy way to make the money and all there in it for.

Well who are the hacks here the people that run 10 or 20 plants that in fact select keep clones of the selected plants use a male test the seed made and then think yes or no if no select threw more seed and find more males and re test its off spring untill there happy with the results or are the hacks really the people you lay prase on that find a female from other peoples work use x chemicals to reverse a female polinate its self or a diffrent female and make seeds in a short time frame slam them on the market and call people who make seed the long yet proven over time method develoved by nature hacks what a fucken joke.

 

[GreenintheThumb]

No one's got gynoecious releases on the market that I would buy...yet.

It's no surprise that the clueless seed companies aren't using the technology properly. It remains, however, a useful tool in good breeding methodologies.

Link to the study showing lower THC please.

 

[JessE]

Slowly going through this mess but I can answer this one.

D This thread has been nothing but lulz for me.

This thread is now full of failure. I'm sorry I haven't had the time to come back until now.

.

Well, well...Dont we think highly of ourselves? You must have pretty low self esteem, or just smoke some shitty herb, who are you trying to convince? Yourself? Or us? Because you're pretending to be king shit in this thread, but you've did what exactly in this community? Thats right, absolutely nothing!

He's on your side.

Boy, for someone pretending to be so knowledgable, you dont know shit do you? He's on Nev's side? LOL! Which side would that be? Against Feminized Seeds? How about doing some research sometime, King Shit?

Mr Watson and his Dutch colleague, biochemist Etienne de Meijer, are confident that by using their own exclusive cross-breeding methods, they can develop healthy plants which will combine only the desired chemical make-up of individual [COLOR=blue !important][COLOR=blue !important]medicines[/color][/color].
There will be no generational deterioration and no genetic difference between each plant because they will be bred from themselves: they will be cloned. "You can clone a plant 10 times," explains Mr de Meijer, "and every time it will be exactly the same."
Mr de Meijer has developed his own technique of "self-progeny" - or "selfing" - where he turns half of one female plant temporarily into a male. Fertilising a plant with itself in this way means the same genetic make-up can be reproduced.
"I can make 20,000 clones with 'selfed' parents in two weeks," he says. "Humans may degenerate from inbreeding, but these plants do not. I'm sure I am the first person to apply this method of inbreeding to cannabis and I found the selfing process was amazingly simple."
But the unique research has no market in Holland. "Because the sale of the drug is tolerated in coffee shops, there is no interest - though people don't really know what they are buying," says Mr Watson.

http://www.independent.co.uk/news/cannabis-a-year-that-changed-minds-1200871.html

. And everything I said to you about cannabis breeding methodologies and the mistakes of the past would apply to him as well as you.

Boy o boy, you're a real clown arent you? You're now in a position to enlighten Skunkman on "breeding methodologies" although you claim that you only wish you've ran Sams numbers?

And I only WISH I've ran Sam's numbers

lmao! So you've got no experience working with real populations? But think you have the answers for those who actually have?

You really think I need your admiration?

So you wished you've ran Sams numbers? But are here pretending you're not after Nevil's admiration, somebody else who has worked with large populations and is a LEGEND in the breeding world? So basically, you're the arm chair expert who has never actually got out of his chair to actually get in the ring? But think you're in the same league as the big boys? lol.

What is it with you clowns who throw your botanical terms around, which most will need a dictionary to decipher? Do you think you're going to trick the layman into thinking you're some kind of expert? Maybe the noobs, who are exactly the ones targeted in the feminization trend scam, but not real veterans in the industry who know better. You can pretend to be king shit all day long, in the end you're just fooling yourself. Not anyone else.

Originally posted by greenthumbuphisbum
Yep, and the few professional breeders who work in our field all feel the same way. Sorry, it's the way biotechnology works in this day and age.

Hilarious! Yes, you (exactly what have you done again? Thats right, nothing!) and the rest of the few professional breeders are all on the same page lmao! When I think about the pro's in the industry with actual credentials, I think Nev, Shanti, Simon and Greenthumbuphisbum...

Wait? Thats right, you're not nearly in any of their leagues. So lets drop the bullshit kid, you're a nothing. You have what? 12 posts? All of them shitty too! Think your 2004 account at Icmag means something? LMAO!! The same Icmag which has perpetuated the Feminization seed scam, via means of the "elite clone" hype? The same Icmag that covered Sams ass when I went to warn the community about his DEA connections? That doesnt strike you as the least bit odd? A known DEA associate, operating at the largest online cannabis forum in the industry? One which gained ground only after all its competitors were raided and forced out? (Sounds familiar dont it Nev?) Yet, you're here pretending to be some expert on feminized seeds and defending their "honor"? The same feminized seed trend capitalized on by the harborers of Skunkman? Hmmm....

I smell a schill boys!

 

[JessE]

Laughing my ass off! He edits his posts here and goes and repost them in a completely new and different thread! AHAHAHA!!!

http://www.mrnice.nl/forum/showthread.php?t=4593

What a sissy!

 

[Kangativa]

No one's got gynoecious releases on the market that I would buy...yet.

It's no surprise that the clueless seed companies aren't using the technology properly. It remains, however, a useful tool in good breeding methodologies.

Link to the study showing lower THC please.

How can it be a useful tool in breeding methodologies when there is only 1 useful tool and that is a big strong healthy male.

Also how can you say that the seed companies arent using this so called
Technology properly, wouldnt you say that after all this time they should have got it right, if that is at all possible ?????

Honestly mate cant you see that all your posts are falling on deaf ears.....I think that you should take all this stuff somewhere where people care, coz from what I have read its not here.

 

[JessE]

No one's got gynoecious releases on the market that I would buy...yet.
.

So Simon, a former College Professor, a man steeped in Science and a true legend in the industry has feminized lines not good enough for you? Who is perhaps the only real breeder today with any kind of true credentials who is offering extensively tested Fem seeds? (Fem seed which still reverses sex with any kind of stress by the way )

You're a complete joke dude! You're in here defending the process, yet nobody has an offering, made with the same process you're defending, that you're willing to buy? lmao!

Should I sit around and wait for your response? How long should I wait, after you've edited them all and start a new thread, until I respond back? Let me know sweet cheeks!

 

[JessE]

Honestly mate cant you see that all your posts are falling on deaf ears.....I think that you should take all this stuff somewhere where people care, coz from what I have read its not here.

But, but, but...Kanga...He's GreenThumbUphisBum lol! This thread would be a joke without him lmao!

 

[GreenintheThumb]

Well, well...Dont we think highly of ourselves? You must have pretty low self esteem, or just smoke some shitty herb, who are you trying to convince? Yourself? Or us? Because you're pretending to be king shit in this thread, but you've did what exactly in this community? Thats right, absolutely nothing!

Can you explain this in your amazing gifs? They're such a boon to the community

Boy, for someone pretending to be so knowledgable, you dont know shit do you? He's on Nev's side? LOL! Which side would that be? Against Feminized Seeds? How about doing some research sometime, King Shit?

Yeah, Sam is ALSO against gynoecious populations. It IS what the thread is about and he IS on his side on the issue.

And yeah, I'm aware of the work that went into the chemovars.

Boy o boy, you're a real clown arent you? You're now in a position to enlighten Skunkman on "breeding methodologies" although you claim that you only wish you've ran Sams numbers?

Enlighten, I doubt. Surly he's heard the arguments before. And most people wished they had run Sam's numbers.

lmao! So you've got no experience working with real populations? But think you have the answers for those who actually have?

Yeah, lets maintain our diverse haze population with 2 individuals! I know not to do that. I guess Sam and Nevil didn't.

So you wished you've ran Sams numbers? But are here pretending you're not after Nevil's admiration, somebody else who has worked with large populations and is a LEGEND in the breeding world? So basically, you're the arm chair expert who has never actually got out of his chair to actually get in the ring? But think you're in the same league as the big boys? lol.

I know Nevil had no answers for me or for my thoughts on the expression of intersex traits in cannabis sativa. Lots of name calling from the big boys, eh?

What is it with you clowns who throw your botanical terms around, which most will need a dictionary to decipher? Do you think you're going to trick the layman into thinking you're some kind of expert? Maybe the noobs, who are exactly the ones targeted in the feminization trend scam, but not real veterans in the industry who know better. You can pretend to be king shit all day long, in the end you're just fooling yourself. Not anyone else.

Just explaining my point. You know so you don't have to explain what a 'double factor' is 1000 times over.

Wait? Thats right, you're not nearly in any of their leagues. So lets drop the bullshit kid, you're a nothing. You have what? 12 posts? All of them shitty too! Think your 2004 account at Icmag means something? LMAO!! The same Icmag which has perpetuated the Feminization seed scam, via means of the "elite clone" hype? The same Icmag that covered Sams ass when his I went to warn the community about his DEA connections? That doesnt strike you as the least bit odd? A known DEA associate, operating at the largest online cannabis forum in the industry?

Still haven't ever seen any REAL evidence of a DEA connection. I only know his name's listed by the DEA as a legit sources to acquire cannabis seeds. But who do you think the DEA can point to to purchase seeds by the very few researchers they let have them? Are they supposed to tell them to ask high schoolers or will they point them to the legal gardens? Besides that all I see is hearsay.

One which gained ground only after all its competitors were raided and forced out? (Sounds familiar dont it Nev?) Yet, you're here pretending to be some expert on feminized seeds and defending their "honor"? The same feminized seed trend capitalized on by the harborers of Skunkman? Hmmm....

I smell a schill boys!

Yeah, everything's Sam's fault. He doesn't answer my questions or debate me, just like you and Nevil. I learned about feminization technologies from hyb, not fucking Sam. He doesn't even like the idea and fears what would happen to the cannabis populations if man wasn't there to maintain them. I think if man is dead who cares if high thc populations of our favorite plant are here for frogs to look at. Obviously we had bigger issues than cannabis if we aren't around.

 

[hempy]

No one's got gynoecious releases on the market that I would buy...yet.

It's no surprise that the clueless seed companies aren't using the technology properly. It remains, however, a useful tool in good breeding methodologies.

Link to the study showing lower THC please.

Plants synthesize an astonishing diversity of isoprenoids, some of which play essential roles in photosynthesis, respiration, and the regulation of growth and development. Two independent pathways for the biosynthesis of isoprenoid precursors coexist within the plant cell: the cytosolic mevalonic acid (MVA) pathway and the plastidial methylerythritol phosphate (MEP) pathway. However, little is known about the effects of plant hormones on the regulation of these pathways. In the present study we investigated the effect of gibberellic acid (GA(3)) on changes in the amounts of many produced terpenoids and the activity of the key enzymes, 1-deoxy-D-xylulose 5-phosphate synthase (DXS) and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), in these pathways. Our results showed GA(3) caused a decrease in DXS activity in both sexes that it was accompanied by a decrease in chlorophylls, carotenoids and Delta(9)-tetrahydrocannabinol (THC) contents and an increase in alpha-tocopherol content. The treated plants with GA(3) showed an increase in HMGR activity. This increase in HMGR activity was followed by accumulation of stigmasterol and beta-sitosterol in male and female plants and campestrol in male plants. The pattern of the changes in the amounts of sterols was exactly similar to the changes in the HMGR activity. These data suggest that GA(3) can probably influence the MEP and MVA pathways oppositely, with stimulatory and inhibitory effects on the produced primary terpenoids in MVA and DXS pathways, respectively.

Effects of gibberellic acid on primary terpenoids and delta-tetrahydrocannabinol in Cannabis sativa at flowering stage.

http://www.ncbi.nlm.nih.gov/pubmed/19522814

 

[GreenintheThumb]

So Simon, a former College Professor, a man steeped in Science and a true legend in the industry has feminized lines not good enough for you? Who is perhaps the only real breeder today with any kind of true credentials who is offering extensively tested Fem seeds? (Fem seed which still reverses sex with any kind of stress by the way )

Chimera and hyb have true credentials too. Funny how their stuff didn't reverse with stress. Gosh, I guess there's a difference between stress testing true females and then inducing a male phenotype and selecting for intersex traits. Do you think maybe there's a difference?

You're a complete joke dude! You're in here defending the process, yet nobody has an offering, made with the same process you're defending, that you're willing to buy? lmao!

Yep, the same way I used to bitch about people using a useful breeding technique known as backcrossing to try to get their clones in seed form.

Should I sit around and wait for your response? How long should I wait, after you've edited them all and start a new thread, until I respond back? Let me know sweet cheeks!

I have only edited posts for spelling, or to clarify a point. Never changed anything big and I never started a thread here.

 

[JessE]

It took you 19 minutes to respond back with this trash? lol. And you still managed to make a complete fool of yourself! And you're riding Skunkmans dick real hard while you're at it too lol.

Why is it you clowns always post one lines, devoid of any content, when I get on your ass? Perhaps if you didnt come to the forum with your shitty attitude, I wouldnt wedge my foot so far up your ass you can read the country of origin my Nike's were made in lol!

Still haven't ever seen any REAL evidence of a DEA connection.

*Yawns*
This is like shooting fish in a barrel.

This is the last time I hold your hand for you little girl, the next time actually do some research before attempting to play with the big boys kid.

Importation would enable U.S. researchers to conduct research on marijuana grown by HortaPharm, a company that has developed unique strains of marijuana. However, no U.S. researcher has imported HortaPharm's marijuana because Dutch authorities have refused to issue an export permit, despite the issuance of an import permit by the DEA (D. Pate, HortaPharm, personal communication, 1998).* HortaPharm, which is in the Netherlands, grows marijuana as a raw material for the manufacture of pharmaceuticals.

http://www.nap.edu/openbook.php?record_id=6376&page=193

What the "National Academies Press" not hard enough evidence of a DEA connection to you clown? lmao!


http://www.nap.edu/openbook.php?record_id=6376&page=193

 

[GreenintheThumb]

Plants synthesize an astonishing diversity of isoprenoids, some of which play essential roles in photosynthesis, respiration, and the regulation of growth and development. Two independent pathways for the biosynthesis of isoprenoid precursors coexist within the plant cell: the cytosolic mevalonic acid (MVA) pathway and the plastidial methylerythritol phosphate (MEP) pathway. However, little is known about the effects of plant hormones on the regulation of these pathways. In the present study we investigated the effect of gibberellic acid (GA(3)) on changes in the amounts of many produced terpenoids and the activity of the key enzymes, 1-deoxy-D-xylulose 5-phosphate synthase (DXS) and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), in these pathways. Our results showed GA(3) caused a decrease in DXS activity in both sexes that it was accompanied by a decrease in chlorophylls, carotenoids and Delta(9)-tetrahydrocannabinol (THC) contents and an increase in alpha-tocopherol content. The treated plants with GA(3) showed an increase in HMGR activity. This increase in HMGR activity was followed by accumulation of stigmasterol and beta-sitosterol in male and female plants and campestrol in male plants. The pattern of the changes in the amounts of sterols was exactly similar to the changes in the HMGR activity. These data suggest that GA(3) can probably influence the MEP and MVA pathways oppositely, with stimulatory and inhibitory effects on the produced primary terpenoids in MVA and DXS pathways, respectively.

Effects of gibberellic acid on primary terpenoids and delta-tetrahydrocannabinol in Cannabis sativa at flowering stage.

http://www.ncbi.nlm.nih.gov/pubmed/19522814

But the point of reversal is to get pollen. You don't fucking smoke those plants. It also doesn't effect the DNA or the progeny. Nice try tho. BTW most people aren't using gibberellic acid anymore.

 

[Kangativa]

Plants synthesize an astonishing diversity of isoprenoids, some of which play essential roles in photosynthesis, respiration, and the regulation of growth and development. Two independent pathways for the biosynthesis of isoprenoid precursors coexist within the plant cell: the cytosolic mevalonic acid (MVA) pathway and the plastidial methylerythritol phosphate (MEP) pathway. However, little is known about the effects of plant hormones on the regulation of these pathways. In the present study we investigated the effect of gibberellic acid (GA(3)) on changes in the amounts of many produced terpenoids and the activity of the key enzymes, 1-deoxy-D-xylulose 5-phosphate synthase (DXS) and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), in these pathways. Our results showed GA(3) caused a decrease in DXS activity in both sexes that it was accompanied by a decrease in chlorophylls, carotenoids and Delta(9)-tetrahydrocannabinol (THC) contents and an increase in alpha-tocopherol content. The treated plants with GA(3) showed an increase in HMGR activity. This increase in HMGR activity was followed by accumulation of stigmasterol and beta-sitosterol in male and female plants and campestrol in male plants. The pattern of the changes in the amounts of sterols was exactly similar to the changes in the HMGR activity. These data suggest that GA(3) can probably influence the MEP and MVA pathways oppositely, with stimulatory and inhibitory effects on the produced primary terpenoids in MVA and DXS pathways, respectively.

Effects of gibberellic acid on primary terpenoids and delta-tetrahydrocannabinol in Cannabis sativa at flowering stage.

http://www.ncbi.nlm.nih.gov/pubmed/19522814

Lol.....Ahhh Hempy what would we do without you
Well done mate !!

 

[shantibaba]

Hi All

seems this thread has hit some people in the nerve endings...Listen I do not mind people differing in opinion it is a norm we must contend with on a daily basis for all things. However let us keep it all civil please and try to not condescend or swear unnecessarily since it ruins the reading for others.

We all get frustrated at times trying to put our case across and be understood but it is better to get frustrated and learn to present something intelligently in a civil way than to sling mud.

So by all means keep quoting sources and defending your case but try to avoid name calling and swearing if you can cheers....Sb

 

[GreenintheThumb]

Still haven't ever seen any REAL evidence of a DEA connection. I only know his name's listed by the DEA as a legit sources to acquire cannabis seeds. But who do you think the DEA can point to to purchase seeds by the very few researchers they let have them? Are they supposed to tell them to ask high schoolers or will they point them to the legal gardens? Besides that all I see is hearsay.

Remember that? I'm done defending Sam, he doesn't respond to my public questions or my private messages. Make the HATE SAM THREAD OF 2010 if you want to. Otherwise try to stay on topic.

 

[JessE]

Can you explain this in your amazing gifs?

 

[JessE]

Hi All

seems this thread has hit some people in the nerve endings...Listen I do not mind people differing in opinion it is a norm we must contend with on a daily basis for all things. However let us keep it all civil please and try to not condescend or swear unnecessarily since it ruins the reading for others.

We all get frustrated at times trying to put our case across and be understood but it is better to get frustrated and learn to present something intelligently in a civil way than to sling mud.

So by all means keep quoting sources and defending your case but try to avoid name calling and swearing if you can cheers....Sb

Do you hear that Green? Thats Shanti calling the dogs off! Perhaps next time you talk to Nevil with a bit more respect?

You've got it Shanti, no problems mate.