G'day, I have a cut of CBD Skunk Haze, acquired from Delta 9 in Canada. Its one of my favorite plants and smells absolutely amazing in flower. The CBD : THC ratio is always around 2:1 in my growing conditions and even though the CBD/THC assay is low it has a really nice effect. I'm keen to preserve these genetics in seed form but despite multiple attempts I using different doses of silver thiosulphate I'm yet to get a single seed. I can get a full reversal with plenty of male flowers formed but they don't produce viable pollen. I have heard that this is typical of some Haze types, is this correct?. I would greatly appreciate any insights or tips on how to go about getting this to work - I'm worried that I will loose my favorite clone and never be able to get it back again. Cheers Snomystic.
CBD crew - CBD SKUNK HAZE
- Thread starter Snomystic
- Start date
Swifty
'23, '24 COE Winner
You could hunt for males in the line and do open pollination of even 1:1 mating or if you find a new female that resembles your current female you could try to reverse that one to pollinate your current female.
Even other strains can be used if you breed them back for several generations to your keeper, evey step does involve prodigy testing to see if you made the right choice.
you can find very usefull info here if you search.
Even other strains can be used if you breed them back for several generations to your keeper, evey step does involve prodigy testing to see if you made the right choice.
you can find very usefull info here if you search.
DannyTwoJoints
2023 COE Winner
Hi! Here's the STS mix ratio that I use:
Silver Thiosulfate Mix 20mM
Silver Thiosulfate is often used to block the action of ethylene in plant cell cultures. Ethylene gas is a hormone that increases during senescence and ripening. It may increase in plant cell cultures due to wounding or presence of auxins. Silver Thiosulfate (STS) blocks the action of ethylene...
www.plantmedia.com
And some directions I've lifted from Khalifa Genetics site:
How and When To Apply STS?
Knowing how and when to apply your STS spray solution is just as important, if not more important than knowing how to make STS.Spray on the nodes and bud sites, not on the leaves.
Spraying on the leaves is useless and is more likely to burn the plants. It will not prevent the reversal but it will cause you to waste a lot of spray solution and time.
The first application should occur when the plants are still in their vegetative cycle. Spray your females 1 day before flipping them to flower. Reversed females take longer to start producing pollen than regular males. Therefore, flip the donor plants about 1 to 3 weeks before flipping the receiver plants to flower. This will help ensure that you don’t miss the optimal pollination window.
Then, spray every 4 days until you see the first male flowers forming. Pure Sativas require 1 application every 3 days whereas you can spray the Indicas just once every 5 days.
Not all strains respond to STS the same way. Some may produce empty pollen sacks. That is why it is highly recommended to spray several female plants in order to increase the odds.
Good luck!
DannyTwoJoints
2023 COE Winner
Found a pic of a sterile pollen sac from a while ago